NCL Method GTA-3
Hep G2 Hepatocyte Glutathione Assay
Listed in Datasets | publication by group NCL Protocols
Version 2.0b - published on 09 Jul 2020 doi:10.17917/EHR9-HB86 - cite this
Licensed under these terms
This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and oxidized glutathione, following treatment with nanoparticle formulations, as part of the NCL in vitro preclinical characterization cascade. A shift from reduced to oxidized glutathione is indicative of oxidative stress, while a decrease in the overall reduced glutathione pool is indicative of conjugative metabolism or impaired synthesis (1). This protocol utilizes a colorimetric method for glutathione determination.
- NCL_Method_GTA-3.pdf(PDF | 695 KB)
Cite this work
Researchers should cite this work as follows:
- Stephan Stern, Timothy Potter, Barry Neun (2020). NCL Method GTA-3. (Version 2.0b). NCI Hub. doi:10.17917/EHR9-HB86
This publication belongs to the NCL Protocols group.
When watching a publication, you will be notified when a new version is released.