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NCL Method GTA-1
09 Jul 2020 | Datasets | Contributor(s):
By Stephan Stern1, Pavan Adiseshaiah1, Timothy Potter1
Leidos Biomedical Research, Inc.
This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade.
https://ncihub.cancer.gov/publications/121/?v=3
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NCL Method ITA-36
25 Jun 2020 | Datasets | Contributor(s):
By Barry Neun, Marina Dobrovolskaia
Detection of naturally occurring antibodies to PEG and PEGylated liposomes in plasma of human donor volunteers
https://ncihub.cancer.gov/publications/162/?v=2
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NCL Method ITA-25
25 Jun 2020 | Datasets | Contributor(s):
By Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IFN in culture supernatants
https://ncihub.cancer.gov/publications/155/?v=2
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NCL Method ITA-24
25 Jun 2020 | Datasets | Contributor(s):
By Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human TNF- in culture supernatants
https://ncihub.cancer.gov/publications/154/?v=2
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NCL Method ITA-23
25 Jun 2020 | Datasets | Contributor(s):
By Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IL-1 in culture supernatants
https://ncihub.cancer.gov/publications/153/?v=2
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NCL Method ITA-22
25 Jun 2020 | Datasets | Contributor(s):
By Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IL-8 in culture supernatants
https://ncihub.cancer.gov/publications/152/?v=2
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NCL Method ITA-18
25 Jun 2020 | Datasets | Contributor(s):
By Timothy Potter, Edward Cedrone, Barry Neun, Marina Dobrovolskaia
Human Leukocyte Proliferation Assay (HuLa)
https://ncihub.cancer.gov/publications/151/?v=2
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NCL Method ITA-17
25 Jun 2020 | Datasets | Contributor(s):
By Timothy Potter, Edward Cedrone, Barry Neun, Marina Dobrovolskaia
Analysis of Nanoparticle effects on Leukocyte Procoagulant Activity
https://ncihub.cancer.gov/publications/150/?v=2
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NCL Method ITA-11
25 Jun 2020 | Datasets | Contributor(s):
By Timothy Potter, Barry Neun, Edward Cedrone, Marina Dobrovolskaia
Measurement of Nanoparticle Effects on Cytotoxic Activity of NK Cells by Label-Free RT-CES System
https://ncihub.cancer.gov/publications/146/?v=2
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NCL Method ITA-10
25 Jun 2020 | Datasets | Contributor(s):
By Timothy Potter, Edward Cedrone, Barry Neun, Marina Dobrovolskaia
Preparation of Human Whole Blood and Peripheral Blood Mononuclear Cell Cultures to analyze nanoparticles potential to induce cytokines in vitro
https://ncihub.cancer.gov/publications/189/?v=1
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NCL Method ITA-9.2
25 Jun 2020 | Datasets | Contributor(s):
By Timothy Potter, Barry Neun, Edward Cedrone, Marina Dobrovolskaia
In vitro Assay for Assessing Nanoparticle effects on Monocyte/Macrophage Phagocytic Function
https://ncihub.cancer.gov/publications/211/?v=1
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NCL Method ITA-4
15 Jun 2020 | Datasets | Contributor(s):
By Barry Neun, Chris McLeland, Timothy Potter, Marina Dobrovolskaia, Suzanne Specht1, Tad Guszcynski1
Leidos Biomedical Research, Inc.
Analysis of Nanoparticle Interaction with Plasma Proteins by 2D PAGE
https://ncihub.cancer.gov/publications/137/?v=2
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NCL Method ITA-3
15 Jun 2020 | Datasets | Contributor(s):
By Barry Neun, Edward Cedrone, Marina Dobrovolskaia
Mouse Granulocyte-Macrophage Colony-Forming Unit Assay
https://ncihub.cancer.gov/publications/136/?v=2
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NCL Method ITA-1
12 Jun 2020 | Datasets | Contributor(s):
By Barry Neun, Edward Cedrone, Marina Dobrovolskaia
Analysis of Hemolytic Properties of Nanoparticles
https://ncihub.cancer.gov/publications/133/?v=2
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CEINT's NanoPHEAT Project : Integrating Exposure and Toxicity Data to Build Risk Forecasting Tools
16 Apr 2020 | Contributor(s):: Mark Wiesner, Jaleesia Amos
The NanoPHEAT tool (Nano Product Hazard and Exposure Assessment Tool) is built to compile dose-response curves from literature, including hundreds of papers, and calculates the estimated exposure to nanoparticles as released from real products, based on some in-house experimental values that we...
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NCL Method STE-2.2
30 Mar 2020 | Datasets | Contributor(s):
By Timothy Potter, Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia
Detection of Bacterial Contamination by Agar Plate Test
https://ncihub.cancer.gov/publications/168/?v=1
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NCL Method STE-2.1
30 Mar 2020 | Datasets | Contributor(s):
By Timothy Potter, Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia
Detection of Microbial Contamination
https://ncihub.cancer.gov/publications/167/?v=1
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NCL Method STE-1.4
30 Mar 2020 | Datasets | Contributor(s):
By Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia
Detection and Quantification of Gram Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations by Kinetic Chromogenic LAL Assay
https://ncihub.cancer.gov/publications/166/?v=1
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NCL Method STE-1.3
30 Mar 2020 | Datasets | Contributor(s):
By Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia
Detection and Quantification of Gram Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations by Gel-Clot LAL Assay
https://ncihub.cancer.gov/publications/165/?v=1
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NCL Method STE-1.2
30 Mar 2020 | Datasets | Contributor(s):
By Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia
Detection and Quantification of Gram Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations by Kinetic Turbidity LAL Assay
https://ncihub.cancer.gov/publications/164/?v=1