Name	Cell Line	ATCC	Source	Organism	Tissue	Tumourigenic	Tumorigenic Detail	Morphology	Age Status(years):number	Ethnicity	Gender	Cytogenetic Analysis	Growth Properties Detail	Growth Properties	Doubling Time(hours):number	Culture Media	Split Ratio	Freeze Media	Comments	References
600MPE	600MPE	N/A	UCSF Gray Lab	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		"DMEM, 10%FBS"		"culture medium 95%; DMSO, 5%"		
AU565	AU565	CRL-2351	ATCC	Homo Sapiens (Human)	Mammary gland			Epithelial	43	Caucasian	Female		Adherent	Adherent		"RPMI 1640, 10%FBS"	1:4 to 1:6		"The AU565 cell line was derived at the Naval Biosciences Laboratory, Oakland, CA from a pleural effusion of a patient with breast carcinoma. This cell line was established from the same patient as SK-BR-3 (ATCC HTB-30). The patient, a White, Caucasian female, age 43, blood type A+, had been treated with radiation, steroids, cytoxan and 5-fluorouracil. The AU565 cell line amplifies and overexpresses the HER-2/neu oncogene; it expresses the HER-3, HER-4 and p53 oncogenes. The cells are useful as models of cellular growth to study the differentiation of breast cancer cells.Treating the cells with mycophenolic acid (MPA), phorbol 12-myristate 13-acetate (PMA), retinoic acid (RA) or the TA-1 monoclonal antibody to the extracellular domain of the HER-2/neu protein can induce cell differentiation. Treatment of AU565 cells with Neu differentiation factor (NDF) also induces mature phenotype, which is characterized by the morphological appearance of the cells. Untreated AU565 cells have compact nuclei with thin cytoplasm while treated cells display a morphology associated with a differentiated phenotype such as flat large nuclei surrounded by a sizable cytoplasm. A culture submitted to the ATCC in April of 1995 was found to be contaminated with mycoplasma and progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative."	"Bacus SS et al. Differentiation of cultured human breast cancer cells (AU-565 and MCF-7) associated with loss of cell surface HER-2/neu antigen. Mol. Carcinog. 3: 350-362, 1990 PubMed: 91119659 Bacus SS et al. Tumor-inhibitory monoclonal antibodies to the HER-2/Neu receptor induce differentiation of human breast cancer cells. Cancer Res. 52: 2580-2589, 1992 PubMed: 92233423 Bacus SS et al. Neu differentiation factor (heregulin) induces expression of intercellular adhesion molecule 1: implications for mammary tumors. Cancer Res. 53: 5251-5261, 1993 PubMed: 94036807 Wen D et al. Neu differentiation factor: a transmembrane glycoprotein containing an EGF domain and an immunoglobulin homology unit. Cell 69: 559-572, 1992 PubMed: 92257596 Bacus SS et al. A ligand for the erbB-2 oncogene product (gp30) induces differentiation of human breast cancer cells. Cell Growth Differ. 3: 401-411, 1992 PubMed: 93041373 Peles E et al. Isolation of the neu/HER-2 stimulatory ligand: a 44 kd glycoprotein that induces differentiation of mammary tumor cells. Cell 69: 205-216, 1992 PubMed: 92208945 Lessor T et al. Regulation of heregulin beta1-induced differentiation in a human breast carcinoma cell line by the extracellular-regulated kinase (ERK) pathway. J. Cell. Biochem. 70: 587-595, 1998 PubMed: 98375973 Yoo JY et al. Inhibition of cell proliferation by 17beta-estradiol and heregulin beta1 in estrogen receptor negative human breast carcinoma cell lines. Breast Cancer Res. Treat. 51: 71-81, 1998 PubMed: 99092857 Yoo JY and Hamburger AW. Changes in heregulin beta1 (HRGbeta1) signaling after inhibition of ErbB-2 expression in a human breast cancer cell line. Mol. Cell. Endocrinol. 138: 163-171, 1998 PubMed: 98348386"
BT20	BT20	HTB-19	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	In nude mice the cells form grade II adenocarcinomas.	Epithelial	74	Caucasian	Female		Adherent	Adherent			1:2 to 1:4	"culture medium 95%; DMSO, 5%"	"This breast tumor line was established by E.Y. Lasfargues and L. Ozzello in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. A mycoplasma contaminant was discovered and eliminated early in 1972. Growth of BT-20 cells is inhibited by tumor necrosis factor alpha (TNF alpha). BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5. The cells express the WNT3 and the WNT7B oncogenes [PubMed: 8168088]."	"Sugarman BJ , et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Lan MS , et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 Castles CG , et al. Expression of a constitutively active estrogen receptor variant in the estrogen receptor-negative BT-20 human breast cancer cell line. Cancer Res. 53: 5934-5939, 1993. PubMed: 8261406 Huguet EL , et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 Lasfargues EY , Ozzello L . Cultivation of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537 Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241"
BT474	BT474	HTB-20	UCSF Gray Lab	Homo Sapiens (Human)	Mammary gland	Yes	"In nude mice, forms nodules in Amsterdam/IMR rats with regressionin 10 days."	Epithelial	60	Caucasian	Female	"The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14;?)(q32,?), unknown, iso(13q), der(6)t(6;7)(q21;q21), der(11)t(11;?)(14;?), del(11)(p11), unknown, unknown, der(2)t(2;?)(p21;?). Several of the latter were reported by E. Lasfargues, et al."	"Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent"	Adherent		"RPMI 1640, 10%FBS"	1:2 to 1:3	"culture medium 95%; DMSO, 5%"	"The BT474 cell line was isolated by E.Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast."	"Lasfargues EY et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978 PubMed: 79029663 Lasfargues EY et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979 PubMed: 80136092 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913"
BT483	BT483	HTB-121	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	Tumorigenic in nude mice; also forms tumors in Amsterdam/IMR rats	Epithelial	23	Caucasian	Female	"mode = 72, range = 46 to 130 "	adherent patches of epithelial cells 	Adherent		"RPMI 1640, 10%FBS"	1:02	"culture medium 95%; DMSO, 5%"	"The BT-483 line was isolated by E.Y. Lasfargues and W.G. Coutinho from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer. The cells were highly pleomorphic with prominent nuclei and nucleoli. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy. BT-483 cells grow in soft agar. Purified DNA from this line."	""Lasfargues EY et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978 PubMed: 79029663 Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992 PubMed: 92233404 ""
BT549	BT549	HTB-122	Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial	72	Caucasian	Female	mode = 74; range = 53 to 140; three marker chromosomes 	Adherent	Adherent		"RPMI 1640, 10%FBS"	1:02	"culture medium 95%; DMSO, 5%"	"The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. The established population was polymorphic with epithelial like components and multinucleated giant cells. A mucin-like material was secreted into the medium."	"Katayose Y et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res. 57: 5441-5445, 1997 PubMed: 98069835 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913"
CAMA1	CAMA1	HTB-21	UCSF Gray Lab	Homo Sapiens (Human)	Mammary gland	Yes	"In nude mice, forms poorly differentiated grade III tumors consistent with breast carcinoma "	Epithelial	51	Caucasian	Female	"modal number = 80; range = 68 to 83; hypertriploid. There are 12 to 13 marker chromosomes per cell; 11 markers were common to all cells, and 7 of them were mostly paired. Among the paired markers were mar(1qter--q21::?::6p11.2--6pter), t(3q::?) and i(16g). Single markers were der(12)t(12;?)(q24:?) and 8 to 9 others. Double minutes occurred in some cells; however, they were present as only 1 or 2 copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells; paired normal X chromosomes were present in every cell. ""	"Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent "	Adherent		"DMEM, 10%FBS"	1:3 to 1:4 	"culture medium 95%; DMSO, 5%"	The CAMA-1 line was established by J. Fogh at Sloan-Kettering in 1975 from cells in the pleural effusion of a patient with carcinoma of the breast. An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.	
HCC1143	HCC1143	CRL-2321	Adi Gazdar S	Homo Sapiens (Human)	Mammary gland			Epithelial	52	Caucasian	Female	polyploid 	Adherent	Adherent		"RPMI 1640, 10% FBS"	1:2 to 1:4	"culture medium 95%; DMSO, 5%"	"HCC1143 is a poorly differentiated cell line that was initiated on August 30, 1994; it took 29 months to establish. HCC1143 was derived from an invasive ductal carcinoma with no lymph node metastases. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC1143 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR)."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1187	HCC1187	CRL-2322	Adi Gazdar	Homo Sapiens (Human)	Mammary gland			Epithelial	41	Caucasian	Female	multiploid; cell population has several ploidy indices 	"mixed, adherent and suspension "	Mixed		"RPMI 1640, 10% FBS"	1:02	"culture medium 95%; DMSO, 5%"	"This cell line was initiated on 9/13/94 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish. The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available. The cells are poorly differentiated. The cells are negative for expression of Her2-neu; they overexpress p53. HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of progesterone receptor (PR). An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1428	HCC1428	CRL-2327	Adi Gazdar	Homo Sapiens (Human)	Mammary gland			Epithelial	49	Caucasian	Female	polyploid	Adherent	Adherent		"RPMI 1640, 10% FBS"	1:4 to 1:8	"culture medium 95%; DMSO, 5%"	"This cell line was initiated on 11/4/95 and took 15 months to establish. HCC1428 was derived from a female who harbored a homozygous deletion in the FHIT gene. The FHIT gene, which spans the FRA3B fragile site at chromosome 3p14.2, is a candidate tumor suppressor gene in breast and other cancers. [32258] There is a familial history of breast cancer (maternal grandmother). The cells are negative for expression of Her2-neu and for expression of p53. HCC1937 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19. An EBV transformed lymphoblastoid cell line [HCC1428 BL] from the same patient is available as ATCC CRL-2328. The homozygous deletion in the FHIT gene is retained in the lymphoblastoid line."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1500	HCC1500	CRL-2329	Adi Gazdar S	Homo Sapiens (Human)	Mammary gland			Epithelial	23	Black	Female	near diploid	Adherent	Adherent		"RPMI 1640, 10% FBS"	1:2 to 1:3	"culture medium 95%; DMSO, 5%"	"This cell line was initiated on 2/8/95 from a patient with a significant family history of early-onset colon cancer as well as a sister with breast cancer. The cell line took 14 months to establish. The tumor was classified as TNM stage IIB, grade 2, invasive ductal carcinoma with 4 out of 24 lymph node metastasis. The cells are poorly differentiated. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC1500 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are positive for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR). HCC1500 contains a homozygous deletion at 3p21.3."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1569	HCC1569	CRL-2330	Adi Gazdar S	Homo Sapiens (Human)	Mammary gland			Epithelial	70	Black	Female	polyploid	Adherent	Adherent		"RPMI 1640, 10% FBS"		"culture medium 95%; DMSO, 5%"	"This cell line was initiated on 3/8/95 and took 19 months to establish. The patient received prior chemotherapy and had no family history of breast cancer. The tumor was classified as TNM stage IV, grade 3, metaplastic carcinoma with 4 out of 18 lymph node metastasis. The cells are poorly differentiated. The cells are positive for expression of Her2-neu and negative for expression of p53. HCC1569 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR) by immunohistochemistry, but PR expression was detectable at a low level by cytosolic protein assay. The cell line is derived from an older patient with a germline mutation in the FHIT gene. The FHIT gene is located at 3p14.2 and the mutation is a transversion at nucleotide 651 (G to T).The patient's daughter carries the same gene mutation. "	"Ahmadian M , et al. Analysis of the FHIT gene and FRA3B region in sporadic breast cancer, preneoplastic lesions, and familial breast cancer probands. Cancer Res. 57: 3664-3668, 1997. PubMed: 9288768 Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1599	HCC1599	CRL-2331	Adi Gazdar F	Homo Sapiens (Human)	Mammary gland			Epithelial	44	Caucasian	Female	multiploid. The cell population has several ploidy indices.	"suspension, multicell aggregates"	Suspension 		"ACL4,  5% FBS"		"culture medium 95%; DMSO, 5%"	"The HCC1599 cell line was initiated on March 28, 1995, and took 10 months to establish. The tumor was classified as a TNM stage IIIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available. The cells are poorly differentiated. The cells are negative for expression of Her2-neu and for expression of p53. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR). HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19. An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1937	HCC1937	CRL-2336	Adi Gazdar F	Homo Sapiens (Human)	Mammary gland			Epithelial	23	Caucasian	Female	"CRL-2336 is highly transformed which is evident from the chromosome count and karyotype description. The modal chromosome number is 100. At least forty-three marker chromosomes, involving nearly every chromosome, were found. Chromosome 1 and chromosome 3 derivative chromosomes were verified using commercial whole chromosome paint (fluorescent in-situ hybridization (FISH)) probes. An acrocentric chromosome with an extra C-band at qter was detected (2 copies per metaphase). There were no normal X chromosomes however at least one derivative X was seen in each cell. The absence of a Y chromosome was verified by QM staining and C-bands. Normal copies of N1, N4, N15, N16 and N18 were absent. More detailed cytogenetic information is available upon request."	The line grows as large epithelial cells with a tendency to float at high cell densities	Suspension 		"ACL4,  5% FBS"	1:2 to 1:4	"culture medium 95%; DMSO, 5%"	"This cell line was initiated from a primary ductal carcinoma on October 13, 1995, and took 11.5 months to establish The tumor was classified as TNM Stage IIB, grade 3 BRCA1 analysis revealed that the cell line is homozygous for the BRCA1 5382C mutation, whereas the lymphoblastoid cell line derived from the same patient is heterozygous for the same mutation This mutation was present in two other family members; an identical sister also developed breast cancer The cell line has an acquired mutation of TP53 with wild-type allele loss; an acquired homozygous deletion of the PTEN gene, and loss of heterozygosity at multiple loci known to be involved in the pathogenesis of breast cancer The cells are negative for expression of Her2-neu and for expression of p53. HCC1937 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19 The cells are negative for expression of estrogen receptor (ER) and progesterone receptor (PR) An EBV transformed lymphoblastoid cell line (HCC1937BL) from the same patient is available as ATCC CRL-2337"	"Tomlinson GE , et al. Characterization of a breast cancer cell line derived from a germ-line BRCA1 mutation. Cancer Res. 58: 3237-3242, 1998. PubMed: 9699648 Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1954	HCC1954	CRL-2338	Adi Gazdar	Homo Sapiens (Human)	Mammary gland			Epithelial	61	East Indian	Female		Adherent	Adherent		"RPMI 1640, 10% FBS"		"culture medium 95%; DMSO, 5%"	"The HCC1954 is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish. HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC202	HCC202	CRL-2316	Adi Gazdar S	Homo Sapiens (Human)	Mammary gland			Epithelial	82	Caucasian	Female	polyploid 	Adherent	Adherent		"RPMI 1640, 10% FBS"		"culture medium 95%; DMSO, 5%"	"The HCC202 cell line was initiated from a primary ductal carcinoma on September 5, 1992, and took 41 months to establish. The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 19 lymph nodes. The cells are poorly differentiated. The cells are positive for expression of Her2-neu, but are negative for expression of p53. HCC202 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -) and progesterone receptor (PR -)."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC2185	HCC2185		Adi Gazdar F	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		"RPMI 1640, 10% FBS"		"culture medium 95%; DMSO, 5%"		
HCC3153	HCC3153		Adi Gazdar	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		"RPMI 1640, 10%FBS"		"culture medium 95%; DMSO, 5%"		
HCC38	HCC38	CRL-2314	Adi Gazdar S&F	Homo Sapiens (Human)	Mammary gland			Epithelial	50	Caucasian	Female	Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% 	"adherent, single cells and loosely attached clusters "	Adherent		"ACL4,  5% FBS"	1:2 to 1:4	"culture medium 95%; DMSO, 5%"	"The cells are negative for expression of Her2-neu but positive for expression of p53 This cell line was initiated on 4/27/92 and took 32 months to establish. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis. HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 Sundarsan V , et al. Homozygous deletions at 3p12 in breast and lung cancer. Oncogene 17: 1723-1729, 1998. PubMed: 9796701"
HCC70	HCC70	CRL-2315	Adi Gazdar S	Homo Sapiens (Human)	Mammary gland			Epithelial	49	Black	Female	polyploid 	Adherent	Adherent		"RPMI 1640, 10% FBS"	1:4 to 1:6	"culture medium 95%; DMSO, 5%"	"The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish. The cells are poorly differentiated. The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 17 lymph nodes. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HS578T	HS578T	HTB-126	Georgetown	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	74	Caucasian	Female	"Number of cells examined = 50; Modal Chromosome Number = 59 with a range of 50 to 77; Polyploidy Rate = 33.8% Composite karyotype: 50-77 <3n> X, -1, del(1)(q12), -2, del(2)(?q36), der(3)t(3;15)(q10;p10),-4, -5, der(5)t(5;8)(p10;q10),-6, i(6)(p10), +8, -9, -10, -11, del(11)(p12), -12, -13, -14, -15, -15, -16, -17, -17, -17, i(17)(q10), -18, -19, der(19)(19pter<-q13::5q13<-qter), +22, +3 mar[cp12] "	Adherent	Adherent		"DMEM, 10%FBS"	1:02	"culture medium 95%; DMSO, 5%"	"The Hs 578T cell strain was derived from a carcinoma of the breast. It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient. The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning. Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy. As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected."	"Hackett AJ et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977 PubMed: 77190209 Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979 PubMed: 79112564 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913"
LY2	LY2		Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		"DMEM, 10%FBS"		"culture medium 95%; DMSO, 5%"		
MCF10A	MCF10A	CRL-10317	Georgetown	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	36		Female	"aneuploid human female; 48, XX, +8, +16, 3p-, 6p+, 9p+"	Adherent	Adherent		DMEM/F12 + goodies	1:3 to 1:4	"culture medium 95%; DMSO, 5%"	"The MCF 10A cell line is a non-tumorigenic epithelial cell line.The line was produced by long term culture in serum free medium with low Ca++ concentration. MCF 10A was derived from adherent cells in the population. Cells derived from a floating population are available (see MCF 10F, ATCC CRL-10318).The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera endotoxin, and epidermal growth factor (EGF).By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.The calcium content of the medium exerts a strong effect on the morphology of the cells."	"Soule H and McGrath CM. Immortal human mammary epithelial cell lines. U.S. Pat. 5,026,637 dated June 25, 1991 Pauley RJ et al. Immortal human mammary epithelial cell sublines. U.S. Pat. 5,206,165 dated Apr. 27, 1993 Soule HD and McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro 22: 6-12, 1986 PubMed: 86111509 Soule HD et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990 PubMed: 90367052 Tait L et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990 PubMed: 90367053"
MCF10A	MCF10A	CRL-10317	Georgetown	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	36		Female	"aneuploid human female; 48, XX, +8, +16, 3p-, 6p+, 9p+"	Adherent	Adherent		DMEM/F12 + goodies	1:3 to 1:4	"culture medium 95%; DMSO, 5%"	"The MCF 10A cell line is a non-tumorigenic epithelial cell line.The line was produced by long term culture in serum free medium with low Ca++ concentration. MCF 10A was derived from adherent cells in the population. Cells derived from a floating population are available (see MCF 10F, ATCC CRL-10318).The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera endotoxin, and epidermal growth factor (EGF).By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.The calcium content of the medium exerts a strong effect on the morphology of the cells."	"Soule H and McGrath CM. Immortal human mammary epithelial cell lines. U.S. Pat. 5,026,637 dated June 25, 1991 Pauley RJ et al. Immortal human mammary epithelial cell sublines. U.S. Pat. 5,206,165 dated Apr. 27, 1993 Soule HD and McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro 22: 6-12, 1986 PubMed: 86111509 Soule HD et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990 PubMed: 90367052 Tait L et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990 PubMed: 90367053"
MCF12A	MCF12A	CRL-10782	Benz's Lab	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	60	Caucasian	Female	range = 65 to 71 	Adherent	Adherent		DMEM/F12 + goodies	1:3 to 1:10	"culture medium 95%; DMSO, 5%"	"The MCF-12A cell line is a non-tumorigenic epithelial cell line established from tissue taken at reduction mammoplasty from a nulliparous patient with fibrocystic breast disease that contained focal areas of intraductal hyperplasia. The line was produced by long term culture in serum free medium with low Ca++ concentration. MCF-12A was derived from adherent cells in the population. Cells derived from a floating population are available (see MCF-12F, ATCC CRL-10783). The cells are positive for epithelial cytokeratins 8, 14 and 18, and negative for cytokeratin 19. They exhibit typical luminal epithelial morphology, three dimensional growth in collagen, and form domes in confluent cultures."	"Pauley RJ et al. Immortal human mammary epithelial cell sublines. U.S. Pat. 5,206,165 dated Apr. 27, 1993 Paine TM et al. Characterization of epithelial phenotypes in mortal and immortal human breast cells. Int. J. Cancer 50: 463-473, 1992 PubMed: 92138085"
MCF7	MCF7	HTB-22	Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial	69	Caucasian	Female	"The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic."	Adherent	Adherent		"DMEM, 10%FBS"	1:3 to 1:6	"culture medium 95%; DMSO, 5%"	The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. Contains the Tx-4 oncogene. Growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF alpha). Secretion of IGFBP's can be modulated by treatment with anti-estrogens.	"Sugarman BJ et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985 PubMed: 86044518 Takahashi K and Suzuki K. Association of insulin-like growth-factor-I-induced DNA synthesis with phosphorylation and nuclear exclusion of p53 in human breast cancer MCF-7 cells. Int. J. Cancer 55: 453-458, 1993 PubMed: 93388025 Brandes LJ and Hermonat MW. Receptor status and subsequent sensitivity of subclones of MCF-7 human breast cancer cells surviving exposure to diethylstilbestrol. Cancer Res. 43: 2831-2835, 1983 PubMed: 83206536 Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990 PubMed: 90242270 Pratt SE and Pollak MN. Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993 PubMed: 94036798 Soule HD et al. A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51: 1409-1416, 1973 PubMed: 74054239 Bellet D et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997 PubMed: 97164677 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913 Komarova EA et al. Intracellular localization of p53 tumor suppressor protein in gamma-irradiated cells is cell cycle regulated and determined by the nucleus. Cancer Res. 57: 5217-5220, 1997 PubMed: 98053886 van Dijk MA et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997 PubMed: 97413630 Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997 PubMed: 97413643 Umekita Y et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996 PubMed: 97030277 Zamora-Leon SP et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996 PubMed: 96312501 Geiger T et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anti-Cancer Drug Des. 13: 35-45, 1998 PubMed: 98134516 Jang SI et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996 PubMed: 96394543 Lee JH et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996 PubMed: 96224044 Chang K and Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996 PubMed: 96133892 Zhu X et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996 PubMed: 96234095 Bacus SS et al. Differentiation of cultured human breast cancer cells (AU-565 and MCF-7) associated with loss of cell surface HER-2/neu antigen. Mol. Carcinog. 3: 350-362, 1990 PubMed: 91119659"
MDAMB134VI	MDAMB134VI	HTB-23	Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial	47	Caucasian	Female	hypodiploid; modal number = 43 with an extra long chromosome 2-like marker; (P28/29) hypodiploid and hypertriploid to hypotetraploid with large subtelocentric marker	monolayer (lightly adherent)	Adherent		"L-15, 20%FBS"	1:2 to 1:4	"culture medium 95%; DMSO, 5%"	"We now use L-15/20% FBS media- this is different from the DMEM/10% FBS stated in our paper. This is one of a series of breast tumor lines (ATCC HTB-23 through 26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973. The cells grow a monolayer of round loosely adherent cells. They grow slowly, becoming heavy (shedding somewhat into the medium) without actually becoming confluent. The cells overexpress FGF receptors, and the fgfr-1 gene is amplified."	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Cruciger Q et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816  McLeskey SW et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994 PubMed: 94101631 Cailleau R et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974 PubMed: 75007579  Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006 RF33081: Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992 PubMed: 92233404  Cruciger QV et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976 PubMed: 77067891"
MDAMB157	MDAMB157	HTB-24	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	Tumorigenic in nude mice and in immunosuppressed BALB/c mice	Epithelial	44	Black	Female	"modal number = 64 to 66 (originator). (P12) Hypotriploid with abnormalities including dicentrics, breaks, large metacentric, submetacentric and subtelocentric markers"	Adherent	Adherent		"DMEM, 10%FBS"	1:2 to 1:3	"culture medium 95%; DMSO, 5%"	"The method for derivation of this line was similar in detail to that for ATCC HTB-23 (MDA-MB-134-VI). Desmosomes, microvilli and tonofilaments were observed at boundaries between cells."	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Cruciger Q et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976 Young RK et al. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro 9: 239-245, 1974 PubMed: 77070455  Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 Cailleau R et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974 PubMed: 75007579 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Cruciger QV et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976 PubMed: 77067891"
MDAMB231	MDAMB231	HTB-26	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	"Tumorigenic in nude mice, also in ALS treated BALB/c mice; forms poorly differentiated adenocarcinoma (grade III)"	Epithelial	51	Caucasian	Female	"The cell line is aneuploid female, with chromosome counts in the near-triploid range. Normal chromosomes N8 and N15 were absent. Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic. Many of the marker chromosomes are identical to those shown in the karyotype reported by K.L. Satya-Prakash, et al."	Adherent	Adherent		"DMEM, 10%FBS"	1:2 to 1:4	"culture medium 95%; DMSO, 5%"	The cell line was isolated as described for ATCC HTB-23. Purified DNA from this line is available as ATCC 45518 (25 micrograms) and ATCC 45519 (100 micrograms).	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Cruciger Q et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 Cailleau R et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974 PubMed: 75007579 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Bates SE et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990 PubMed: 90092004 Dickstein B et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J. Cell. Physiol. 157: 110-118, 1993 PubMed: 94012995 Huguet EL et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994 PubMed: 94221588 Satya-Prakash KL et al. Cytogenetic analysis on eight human breast tumor cell lines: high frequencies of 1q, 11q and HeLa-like marker chromosomes. Cancer Genet. Cytogenet. 3: 61-73, 1981 PubMed: 82001960 Katayose Y et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res. 57: 5441-5445, 1997 PubMed: 98069835 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913 Sheng S et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996 PubMed: 97030253 De Vincenzo R et al. Antiproliferative activity of colchicine analogues on MDR-positive and MDR-negative human cancer cell lines. Anti-Cancer Drug Des. 13: 19-33, 1998 PubMed: 98134515 Soker S et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996 PubMed: 96215040"
MDAMB361	MDAMB361	HTB-27	Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial	40	Caucasian	Female	"(P40) hyperdiploid to hypotriploid with abnormalities including minutes and double minutes (DM), large subtelocentric chromosome and large submetacentric marker"	monolayer (loosely attached)	Adherent		"DMEM, 10%FBS"	1:2 to 1:6	"culture medium 95%; DMSO, 5%"	This line differs from others of the series (ATCC HTB-23 to 26) in karyology and in that it was isolated from a brain metastasis.	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Cruciger Q et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 Cailleau R et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974 PubMed: 75007579 Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992 PubMed: 92233404 Huguet EL et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994 PubMed: 94221588"
MDAMB415	MDAMB415	HTB-128	Georgetown	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	38	Caucasian	Female	"The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations."	Adherent	Adherent		"DMEM, 10%FBS"	1:02	"culture medium 95%; DMSO, 5%"	"The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells. The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization. Purified DNA from this line is available as ATCC 45524 (25 micrograms) and ATCC 45525."	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Beall PT et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979 PubMed: 81064293 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Huguet EL et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994 PubMed: 94221588"
MDAMB436	MDAMB436	HTB-130	Georgetown	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	43	Caucasian	Female	modal number = 45	Adherent	Adherent		"L15, 10%FBS"	1:02	"culture medium 95%; DMSO, 5%"	The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990 PubMed: 90242270"
MDAMB453	MDAMB453	HTB-131	Georgetown	Homo Sapiens (Human)	Mammary gland	No	Did form colonies in semisolid medium. 	Epithelial	48	Caucasian	Female		Adherent	Adherent		"DMEM, 10%FBS"	1:2 to 1:6	"culture medium 95%; DMSO, 5%"	"MDA-MB-453 was derived in 1976 by R. Cailleau et al. from an effusion of a 48 year old female patient with metastatic carcinoma of the breast, involving the nodes, brain and both pleural and pericardial cavities. The cells overexpress FGF receptors."	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 McLeskey SW et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994 PubMed: 94101631 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Soker S et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996 PubMed: 96215040 Noonberg SB et al. Evidence of post-transcriptional regulation of U6 small nuclear RNA. J. Biol. Chem. 271: 10477-10481, 1996 PubMed: 96209965"
MDAMB468	MDAMB468	HTB-132	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.	Epithelial	51	Black	Female	"The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range. Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line. A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes. Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype. "	Adherent	Adherent		"L15, 10%FBS"	1:2 to 1:4	"culture medium 95%; DMSO, 5%"	"The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast.Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. EGF receptor is present at 1 X 10 exp6 per cell. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution."	"Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980 PubMed: 81042058 Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979 PubMed: 79146816 Pathak S et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979 PubMed: 79112569 Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978 PubMed: 79087497 Nigro JM et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989 PubMed: 90081846 Bates SE et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990 PubMed: 90092004 Avila MA et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994 PubMed: 94215181 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913  Zamora-Leon SP et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996 PubMed: 96312501"
HBL100	HBL100	HBL100	UCSF Gray Lab	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		"DMEM, 10%FBS"		"culture medium 95%; DMSO, 5%"		
SUM190PT	SUM190PT	SUM190PT	Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-SF-IH		"culture medium 95%; DMSO, 5%"		
HCC2157	HCC2157	HCC2157	Adi Gazdar F	Homo Sapiens (Human)	Mammary gland			Epithelial	48	Black	Female	Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% 	Cells in suspension show an inclination to form duct-like structures and gland-like structures	Suspension 		"RPMI 1640, 10% FBS"		"culture medium 95%; DMSO, 5%"	"This cell line was initiated on 3/4/96 and took 8 months to establish. The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). The cells are positive for expression of Her2-neu and for expression of p53. HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
HCC1007	HCC1007	HCC1007	Adi Gazdar S&F	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Non-Adherent	Non-Adherent		"ACL4,  5% FBS"		"culture medium 95%; DMSO, 5%"		
HCC1008	HCC1008	HCC1008	Adi Gazdar F	Homo Sapiens (Human)	Mammary gland			Epithelial	67	Black	Female	multiploid; cell population has several ploidy indices; double minute (DM) chromosomes were observed	Non-Adherent	Non-Adherent		"ACL4,  5% FBS"		"culture medium 95%; DMSO, 5%"	"This cell line was initiated from an axillary lymph node on 6/7/94 and took 12.5 months to establish. The tumor was classified as TNM stage IIA, grade 3, ductal carcinoma with 12 out of 12 lymph node metastasis. The cells are positive for expressions of Her2-neu and p53 oncogenes. HCC1008 is positive for the epithelial cell specific marker, Epithelial Glycoprotein 2 (EGP2) and cytokeratin 19. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR)."	"Gazdar AF , et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771"
MDAMB435	MDAMB435	MDAMB435	Georgetown	Homo Sapiens (Human)	Skin			Epithelial			Female		Adherent	Adherent		"DMEM, 10%FBS"		"culture medium 95%; DMSO, 5%"	"There is debate regarding whether this is a breast cancer cell lines or not, since it clustered with melanoma samples in PMID: 12354931, 15679052, 15150101.A recent article by Rae et al (PMID 17004106) definitively identifies this as a line derived from the M14 melanoma cell line.For more information see: http://dtp.nci.nih.gov/docs/misc/common_files/mda-mb-435-update.html"	
DU4475	DU4475	DU4475	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	In nude mice; forms transplantable tumors similar in histology to that of the original carcinoma 	Epithelial	70	Caucasian	Female	mode = 93; range = 78 to 96; six marker chromosomes 	suspension 	Suspension 		"RPMI 1640, 10%FBS"		"culture medium 95%; DMSO, 5%"	Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy. This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests.	"Langlois AJ et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979 PubMed: 79189511 Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992 PubMed: 92233404"
SKBR3	SKBR3	HTB-30	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	In nude mice; forms poorly differentiated adenocarcinoma	Epithelial	43	Caucasian	Female	"This is a hypertriploid human cell line with the modal chromosome number of 84, occurring in 34% of cells. Cells having 80 chromosomes also occurred at a high rate (28%); the higher ploidy cells occurred at 7.3%. This cell line has a very complex chromosome composition. Thirty-five to 40% of chromosomes in a cell complement with a modal chromosome number of 84 consisted of structurally altered marker chromosomes. Several markers are longer than chromosome N1. The origins of most of these markers, however, are not clear. Some markers may have at least three individual chromosome segments. The markers [i.e., ?der(1)t(1;21) (p13;q21) [or ?t(1q21q)], ?del(2) (q13), and t(7pter--cen--?), present in some cells only] were the only ones in which portions of chromosome segments could be identified. Most cells had about three normal X chromosomes and five or more N7. The structurally normal N1, N14 and N17 were generally absent."	Adherent	Adherent		"McCoys5A, 10%FBS"	1:02	"culture medium 95%; DMSO, 5%"	"This cell line was derived by G. Trempe and L.J. Old in 1970 from a pleural effusion. No virus particles. Purified DNA from this line is available as ATCC 45520 (25 micrograms) and ATCC 45521 (100 micrograms). Ultrastructural features include microvilli and desmosomes, glycogen granules, large lysosomes, bundles of cytoplasmic fibrils. The SK-BR-3 cell line overexpresses the HER2/c-erb-2 gene product."	"Fogh, J., ed., Human tumor cells in vitro. New York: Plenum Press; 1975:pp. 115-159 Trempe GL. Human breast cancer in culture. Recent Results Cancer Res. : 33-41, 1976 PubMed: 77103992 Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006 Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed: 77210034 Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981 PubMed: 81218998 Hudziak RM et al. Monoclonal antibodies directed to the Her2 receptor. U.S. Pat. 5,677,171 dated Oct. 14, 1997 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913 Chavany C et al. p185erbB2 binds to GRP94 in vivo. J. Biol. Chem. 271: 4974-4977, 1996 PubMed: 96214925"
SUM1315MO2	SUM1315MO2		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
SUM149PT	SUM149PT		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
SUM159PT	SUM159PT		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
SUM185PE	SUM185PE		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
SUM225CWN	SUM225CWN		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
SUM44PE	SUM44PE		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
SUM52PE	SUM52PE		Steve Either	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		Ham's F12-5%-IE		"culture medium 95%; DMSO, 5%"		
T47D	T47D	HTB-133	Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial	54		Female	"This is a hypotriploid human cell line. The modal chromosome number is 65 occurring at 50% and polyploidy at 0.8%. 18 marker chromosomes are common to most cells, of which 7 are paired and 11 are single-copied. The t(8q14q), t(9q17q), t(10q17p) are among 7 paired markers common to most cells. N7, N9, and N10 are absent and N11 is generally present in 4 copies. DM's occurred, but infrequently. Q-band examination did not show the presence of a Y chromosome."	Adherent	Adherent		"RPMI 1640, 10%FBS"	1:3 to 1:5	"culture medium 95%; DMSO, 5%"	"The T-47 line was isolated by I. Keydar from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast. This differentiated epithelial substrain (T-47D) was found to contain cytoplasmic junctions and receptors to 17 beta estradiol, other steroids and calcitonin. It will form colonies in soft agar. Purified DNA from this line is available as ATCC 45528 (25 micrograms) and ATCC 45529 (100 micrograms)."	"Keydar I et al. Establishment and characterization of a cell line of human breast carcinoma origin. Eur. J. Cancer 15: 659-670, 1979 PubMed: 80068971 Judge SM and Chatterton RT Jr. Progesterone-specific stimulation of triglyceride biosynthesis in a breast cancer cell line (T-47D). Cancer Res. 43: 4407-4412, 1983 PubMed: 83259030 Lamp SJ et al. Calcitonin induction of a persistent activated state of adenylate cyclase in human breast cancer cells (T-47D). J. Biol. Chem. 256: 12269-12274, 1981 PubMed: 82053097 Sher E et al. Whole-cell uptake and nuclear localization of 1,25-dihydroxy-cholecalciferol by breast cancer cells (T-47D) in culture. Biochem. J. 200: 315-320, 1981 PubMed: 82182106 Freake HC et al. 1,25-Dihydroxyvitamin D3 specifically binds to a human breast cancer cell line (T-47D) and stimulates growth. Biochem. Biophys. Res. Commun. 101: 1131-1138, 1981 PubMed: 82068379 Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992 PubMed: 92233404 Huguet EL et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994 PubMed: 94221588 Bellet D et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997 PubMed: 97164677 Hoppe HC et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997 PubMed: 97427981 Burfeind P et al. Antisense RNA to the type I insulin-like growth factor receptor suppresses tumor growth and prevents invasion by rat prostate cancer cells in vivo. Proc. Natl. Acad. Sci. USA 93: 7263-7268, 1996 PubMed: 96293512"
UACC812	UACC812	CRL-1897	UCSF Gray Lab	Homo Sapiens (Human)	Mammary gland		range = 58 to 64; abnormal banding region in 3p	Epithelial	43		Female	range = 58 to 64; abnormal banding region in 3p	Adherent	Adherent	100	"DMEM, 10%FBS"	1:2 to 1:3	"culture medium 95%; DMSO, 5%"	"This cell line was established by A. Liebovitz and associates in 1986 from breast tissue removed at mastectomy to remove a ductal carcinoma (stage II, grade IV). Prior to surgery, the patient had received extensive chemotherapy. The cells exhibit a 15 fold amplification of the HER-2/neu oncogene sequence. They are negative for estrogen receptors, progesterone receptors and P glycoprotein. The cells grow very slowly, and growth can be enhanced by using 20% fetal bovine serum and adding epidermal growth factor (10 ng/ml) to the medium. Purified DNA from this line is available as ATCC 45534 (25 micrograms) and ATCC 45535 (100 micrograms)."	"Proc. Am. Assoc. Cancer Res. 29: 24, 1988 Meltzer P et al. Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Br. J. Cancer 63: 727-735, 1991 PubMed: 91248618"
ZR751	ZR751	CRL-1500	Georgetown	Homo Sapiens (Human)	Mammary gland	Yes	Forms tumors in nude mice	Epithelial	63	Caucasian	Female	"This human cell line has a hypertriploid chromosome number. The modal chromosome number was 72 occurring in 26% of the cells and the rate of higher ploidies was at 1.2%. Eighteen markers were common. They are: t(1q,?), M2,M3, del(3) (p21), der(5)t(5;?) (q35;?), del(6) (q21), t(11q14q), t(11;11) (p15;q11), der(14)t(2;14) (q21;q32), t(17q,?), M13, M14, M15, M16, M17, der(8)t(8;?), t(9p,?) and 19pt. Of these M13 was paired. Normal N14 was not found. N6 was single copied, X had 3 copies and N18 had 4 copies in each cell."	Adherent	Adherent	80	"RPMI 1640, 10%FBS"	1:4 to 1:6	"culture medium 95%; DMSO, 5%"	"The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.Purified DNA from this line is available as ATCC 45530 (25 micrograms) and ATCC 45531 (100 micrograms)."	"Engel LW and Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978 PubMed: 79022930 Engel LW et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978 PubMed: 79001597 Dahiya R et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993 PubMed: 93185091 Bellet D et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997 PubMed: 97164677 Couillard S et al. Comparison of the effects of the antiestrogens EM-800 and tamoxifen on the growth of human breast ZR-75-1 cancer xenografts in nude mice. Cancer Res. 58: 60-64, 1998 PubMed: 98086027 Wang E et al. Posttranscriptional regulation of protein expression in human epithelial carcinoma cells by adenine-uridine-rich elements in the 3'-untranslated region of tumor necrosis factor-alpha messenger RNA. Cancer Res. 57: 5426-5433, 1997 PubMed: 98053920 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913 Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997 PubMed: 97413643 Sheng S et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996 PubMed: 97030253"
ZR7530	ZR7530	CRL-1504	Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial	47	Black	Female		Adherent	Adherent	110	"RPMI 1640, 10%FBS"	1:2 to 1:3	"culture medium 95%; DMSO, 5%"	Purified DNA from this line is available as ATCC 45532 (25 micrograms) and ATCC 45533 (100 micrograms).	"Engel LW and Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978 PubMed: 79022930 Engel LW et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978 PubMed: 79001597 Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997 PubMed: 98053913"
ZR75B	ZR75B		Georgetown	Homo Sapiens (Human)	Mammary gland			Epithelial			Female		Adherent	Adherent		"RPMI 1640, 10%FBS"	1:2 to 1:3	"culture medium 95%; DMSO, 5%"