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NCL Method ITA-37.2

By Hannah Newton, Jenny Zhang, Marina Dobrovolskaia

Immunophenotyping: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 17 Oct 2022 doi:10.17917/8BPC-VN29 - cite this

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Description

Immunophenotyping is the use of antigen expression for the identification of distinct immune cell subsets (and their activation statuses). This technique can detect minute changes in cell populations and thus is used to characterize the cell makeup in many diseases as well as determine effects of treatments, such as nanoparticles. It is important to develop a method that allows for immunological evaluation of nanoparticles because some nanoparticles are designed to modify the immune system while others cause immunotoxicity. Currently, the most common technique used to perform immunophenotyping is multicolor flow cytometry.

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