NCL Method ITA-37.1
Immunophenotyping: Instrument Calibration and Reagent Qualification for Immunophenotyping Analysis of Human Peripheral Blood Mononuclear Cell Cultures
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Version 1.0 - published on 17 Oct 2022 doi:10.17917/GJ0C-JY85 - cite this Last public release: 2.0
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Description
Immunophenotyping is the use of antigen expression for the identification of distinct immune cell subsets (and their activation statuses). This technique can detect minute changes in cell populations and thus is used to characterize the cell makeup in many diseases as well as determine effects of treatments, such as nanoparticles. It is important to develop a method that allows for immunological evaluation of nanoparticles because some nanoparticles are designed to modify the immune system while others cause immunotoxicity. Currently, the most common technique used to perform immunophenotyping is multicolor flow cytometry.
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- NCL_Method_ITA-37.1.pdf(PDF | 790 KB)
Cite this work
Researchers should cite this work as follows:
- Newton, H., Zhang, J., Marina Dobrovolskaia (2022). NCL Method ITA-37.1. NCI Hub. doi:10.17917/GJ0C-JY85
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NCL Protocols
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