NCL Method ITA-37.1
Immunophenotyping: Instrument Calibration and Reagent Qualification for Immunophenotyping Analysis of Human Peripheral Blood Mononuclear Cell Cultures
Listed in Datasets | publication by group NCL Protocols
Version 2.0 - published on 14 Mar 2024 doi:10.17917/8FKW-D647 - cite this
Licensed under these terms
Description
Immunophenotyping is the use of antigen expression for the identification of distinct immune cell subsets (and their activation statuses). This technique can detect minute changes in cell populations and thus is used to characterize the cell makeup in many diseases as well as determine effects of treatments, such as nanoparticles. It is important to develop a method that allows for immunological evaluation of nanoparticles because some nanoparticles are designed to modify the immune system while others cause immunotoxicity. Currently, the most common technique used to perform immunophenotyping is multicolor flow cytometry.
Content List
NCL_Method_ITA-37.1.pdf(PDF | 790 KB)
Cite this work
Researchers should cite this work as follows:
- Hannah Newton, Jenny Zhang, Marina Dobrovolskaia (2022). NCL Method ITA-37.1. (Version 2.0). NCI Hub. doi:10.17917/8FKW-D647
Tags
NCL Protocols
This publication belongs to the NCL Protocols group.
When watching a publication, you will be notified when a new version is released.