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NCL Method ITA-14

By Timothy Potter, Edward Cedrone, Barry Neun, Susan Strobl, Liubov Zaritskaya, Marina Dobrovolskaia

Analysis of Nanoparticle Effects on Maturation of Monocyte Derived Dendritic Cells In Vitro

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 14 Jun 2021 doi:10.17917/AJ1Z-2E64 - cite this

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Description

Dendritic cells (DC) are antigen-presenting cells. They play an important role in initiation and regulation of the cellular immune response. Maturation of dendritic cells is achieved by inflammatory cytokines (e.g. TNFa) or pathogen associated molecular patterns (e.g. bacterial lipopolysaccharide, LPS). This protocol can be used to study the in vitro efficacy of nanoparticle-based vaccine formulations. In addition, it can also be used to assess the potential cytotoxicity of nanoparticle formulations to dendritic cells, and the nanoparticle effects on DC maturation in response to traditional inflammatory stimuli such as bacterial lipopolysaccharide.

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