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NCL Method ITA-8.2

By Edward Cedrone, Julia Bui, Naichen Yu, Marina Dobrovolskaia

Analysis of nanoparticle chemoattractant properties using label-free, real-time chemotaxis assay

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 09 Jul 2020 doi:10.17917/CFBS-5C02 - cite this Last public release: 2.0

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This document provides a protocol for a real-time, quantitative assessment of the chemoattractant capacity of engineered nanomaterials.  Leukocyte recruitment is a central component of the inflammatory process in both host defense against pathogens and a range of inflammatory disorders. Leukocytes respond to pro-inflammatory stimuli, including but not limited to cytokines, chemokines, and prostaglandins, by migrating from the bloodstream to the site of inflammation. 

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