NCL Method ITA-24
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human TNF-α in culture supernatants
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Version 2.0b - published on 24 Jul 2020 doi:10.17917/JQM0-0G48 - cite this
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Description
TNF-a is a pro-inflammatory chemokine produced by various types of cells in response to inflammatory stimulus, e.g., bacterial lipopolysaccharide (LPS). This document describes an experimental procedure for analysis of cell culture supernatants for the presence of TNF-a using an enzyme linked immunosorbent assay (ELISA). Refer to NCL protocol ITA-10 for details regarding culture supernatant preparation.
Content List
- NCL_Method_ITA-24_June2020.pdf(PDF | 488 KB)
Cite this work
Researchers should cite this work as follows:
- Barry Neun, Jamie Rodriguez, Timothy Potter, Anna Ilinskaya, Marina Dobrovolskaia (2020). NCL Method ITA-24. (Version 2.0b). NCI Hub. doi:10.17917/JQM0-0G48
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NCL Protocols
This publication belongs to the NCL Protocols group.
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