TNF-a is a pro-inflammatory chemokine produced by various types of cells in response to inflammatory stimulus, e.g., bacterial lipopolysaccharide (LPS). This document describes an experimental procedure for analysis of cell culture supernatants for the presence of TNF-a using an enzyme linked immunosorbent assay (ELISA). Refer to NCL protocol ITA-10 for details regarding culture supernatant preparation.
- NCL_Method_ITA-24_Sep2015.pdf(PDF | 162 KB)
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