NCL Method ITA-23
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IL-1 in culture supernatants
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Version 2.0 - published on 09 Jul 2020 doi:10.17917/BHQ7-GD40 - cite this Last public release: 2.0b
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Description
IL-1b is a pro-inflammatory cytokine produced by various types of cells in response to inflammatory stimulus, e.g. bacterial lipopolysaccharide LPS. This document describes experimental procedure for analysis of culture supernatants by ELISA to detect presence of IL-1b. NCL protocol ITA-10 should be referred to for details of preparation of culture supernatants.
Content List
NCL_Method_ITA-23_June2020.pdf(PDF | 545 KB)
Cite this work
Researchers should cite this work as follows:
- Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia (2020). NCL Method ITA-23. (Version 2.0). NCI Hub. doi:10.17917/BHQ7-GD40
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NCL Protocols
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