NCL Method ITA-12
Analysis of Nanoparticle Effects on Plasma Coagulation Times in Vitro
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 04 May 2020 doi:10.17917/P6SK-3R71 - cite this Last public release: 2.0b
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Description
This document describes a protocol for assessing the effect a nanoparticle formulation may have on plasma coagulation time. Coagulation, i.e. blood clotting, is a highly complex process that involves many components. There are three main pathways for coagulation: intrinsic (also known as the contact activation pathway, because it is activated by a damaged surface); extrinsic (also known as the tissue factor pathway); and the final common pathway. Each pathway can be assessed by a specialized test. For example, the activated partial thromboplastin time (APTT) assay is used to assess the intrinsic pathway, while the prothrombin time (PT) assay is a measure of the extrinsic pathway. Extrinsic and intrinsic pathways converge into the common pathway. Thrombin time (TT) is an indicator of the functionality of the final common pathway. Each pathway involves many coagulation factors, some of which overlap between pathways. The APTT assay assesses functionality of factors XII, XI, IX, VIII, X, V, and II. The PT assay assesses activity of factors VII, X, V and II. All three assays assess the role of fibrinogen.
Content List
- NCL_Method_ITA-12_Sep2015.pdf(PDF | 126 KB)
Cite this work
Researchers should cite this work as follows:
- Barry Neun, Jamie Rodriguez, Anna Ilinskaya, Marina Dobrovolskaia (2020). NCL Method ITA-12. NCI Hub. doi:10.17917/P6SK-3R71
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NCL Protocols
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