NCL Method ITA-7
Detection of Nitric Oxide Production by the Macrophage Cell Line RAW264.7
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Version 1.0 - published on 04 May 2020 doi:10.17917/Q94Q-6B29 - cite this
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Description
This document describes a protocol for quantitative determination of nitrite (NO2-), a stable oxidative end-product of the antimicrobial effector molecule nitric oxide in cell culture medium [1, 2]. The protocol is used to evaluate the capability of nanomaterials to induce nitric oxide production by macrophages. Nitric oxide secreted by macrophages has a half-life of seconds; it interacts with a number of different molecular targets, resulting in cytotoxicity. In the presence of oxygen and water, nitric oxide interacts with itself to generate other reactive nitrogen oxide intermediates and ultimately decomposes to form nitrite (NO2−) and nitrate (NO3−).
Content List
- NCL_Method_ITA-7_Sept2015.pdf(PDF | 152 KB)
Cite this work
Researchers should cite this work as follows:
- Barry Neun, Timothy Potter, Jamie Rodriguez, Anna Ilinskaya, Marina Dobrovolskaia (2020). NCL Method ITA-7. NCI Hub. doi:10.17917/Q94Q-6B29
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NCL Protocols
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