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NCL Method GTA-14

By Stephan Stern, Barry Neun

Hep G2 Hepatocarcinoma Homogeneous Apoptosis Assay

Listed in Datasets | publication by group NCL Protocols

Version 2.0 - published on 09 Jul 2020 doi:10.17917/95VC-ZP26 - cite this

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This protocol describes the monitoring of nanoparticle treated human hepatocarcinoma cells (HepG2) for apoptosis, as part of the in vitro NCL preclinical characterization cascade (1, 2).  The protocol utilizes a fluorescent method to measure Caspase-3/7 activation.  This is a homogeneous assay that does not require cell isolation, as required for HepG2 Hepatocarcinoma Apoptosis Assay (NCL Method GTA-6).

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