NCL Method GTA-7
Hepatocyte Primary ROS Assay
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 09 Mar 2020 doi:10.17917/PP52-SF51 - cite this Last public release: 2.0
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Description
This protocol describes the testing of nanoparticle formulations for reactive oxygen species (ROS) generation in male Sprague-Dawley (SD) primary hepatocytes, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent redox active probe. Primary hepatocytes were chosen since they have a greater metabolic activity than hepatocyte cell lines.
Content List
NCL_Method_GTA-7.pdf(PDF | 353 KB)
Cite this work
Researchers should cite this work as follows:
- Stephan Stern, Zolnik, B. (2020). NCL Method GTA-7. NCI Hub. doi:10.17917/PP52-SF51
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NCL Protocols
This publication belongs to the NCL Protocols group.
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