NCL Method GTA-7
Hepatocyte Primary ROS Assay
Listed in Datasets | publication by group NCL Protocols
Version 2.0 - published on 09 Jul 2020 doi:10.17917/0WF6-GX40 - cite this
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This protocol describes the testing of nanoparticle formulations for reactive oxygen species (ROS) generation in male Sprague-Dawley (SD) primary hepatocytes, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent redox active probe. Primary hepatocytes were chosen since they have a greater metabolic activity than hepatocyte cell lines.
- NCL_Method_GTA-7.pdf(PDF | 675 KB)
Cite this work
Researchers should cite this work as follows:
- Stephan Stern, Banu Zolnik (2020). NCL Method GTA-7. (Version 2.0). NCI Hub. doi:10.17917/0WF6-GX40
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