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NCL Method GTA-5

By Stephan Stern, Timothy Potter

LLC-PK1 Kidney Cell Apoptosis Assay

Listed in Datasets | publication by group NCL Protocols

Version 2.0 - published on 09 Jul 2020 doi:10.17917/E9YS-N327 - cite this

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This protocol describes the monitoring of nanoparticle treated porcine renal proximal tubule cells (LLC-PK1) for apoptosis, as part of the in vitro NCL preclinical characterization cascade.  The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.

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