NCL Method GTA-2
Hep G2 Hepatocarcinoma Cytotoxicity Assay
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 09 Mar 2020 doi:10.17917/BFZH-QN35 - cite this Last public release: 2.0b
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Description
This protocol describes the cytotoxicity testing of nanoparticle formulations in human hepatocarcinoma cells (Hep G2), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.
Content List
NCL_Method_GTA-2.pdf(PDF | 645 KB)
Cite this work
Researchers should cite this work as follows:
- Stephan Stern, Pavan Adiseshaiah, Timothy Potter (2020). NCL Method GTA-2. NCI Hub. doi:10.17917/BFZH-QN35
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NCL Protocols
This publication belongs to the NCL Protocols group.
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