NCL Method GTA-1
This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade.
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Version 2.0b - published on 09 Jul 2020 doi:10.17917/Q6EC-XC97 - cite this
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Description
This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.
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NCL_Method_GTA-1.pdf(PDF | 682 KB)
Cite this work
Researchers should cite this work as follows:
- Stephan Stern, Pavan Adiseshaiah, Timothy Potter (2020). NCL Method GTA-1. (Version 2.0b). NCI Hub. doi:10.17917/Q6EC-XC97
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NCL Protocols
This publication belongs to the NCL Protocols group.
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