Mycoplasma is a form of bacteria that lacks a nucleus and a cell wall, and are thus unaffected by many antibiotics. Nanoparticles submitted to the NCL may be subjected to testing for mycoplasma when deemed necessary. The types of nanoparticle...
The NCL does not perform mycoplasma testing in our laboratory. Rather, this test is
outsourced to another department within the Frederick National Laboratory for Cancer Research,
the Protein Expression Laboratory (PEL), within the Cancer Research Technology Program.
Briefly, the NCL will culture nanoparticles with cells for the initial 24 hours, then
passage these cells twenty five (25) times. The supernatants from the final culture will then be
transported to PEL for mycoplasma testing via the VenorGem Mycoplasma PCR-based
Detection Kit from Minerva Biolab
- NCL_Method_STE-3_Sep2015.pdf(PDF | 114 KB)